A novel antibody for human induced pluripotent stem cells and embryonic stem cells recognizes a type of keratan sulfate lacking oversulfated structures.

@article{Kawabe2013ANA,
  title={A novel antibody for human induced pluripotent stem cells and embryonic stem cells recognizes a type of keratan sulfate lacking oversulfated structures.},
  author={K. Kawabe and Daiki Tateyama and H. Toyoda and N. Kawasaki and N. Hashii and Hiromi Nakao and S. Matsumoto and M. Nonaka and H. Matsumura and Yoshinori Hirose and Ayaha Morita and Madoka Katayama and M. Sakuma and M. Furue and T. Kawasaki},
  journal={Glycobiology},
  year={2013},
  volume={23 3},
  pages={
          322-36
        }
}
We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cell lysates gave a single but an unusually diffuse band at a position corresponding to >250 kDa. The antigen protein was isolated from the induced pluripotent stem (iPS) cell lysates… Expand
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