A novel and highly efficient method for genetic transformation of fungi employing shock waves.

@article{MagaaOrtz2013ANA,
  title={A novel and highly efficient method for genetic transformation of fungi employing shock waves.},
  author={Denis Maga{\~n}a-Ort{\'i}z and Nancy Coconi-Linares and Elizabeth Ort{\'i}z-V{\'a}zquez and Francisco Fern{\'a}ndez and Achim M. Loske and Miguel Angel G{\'o}mez-Lim},
  journal={Fungal genetics and biology : FG & B},
  year={2013},
  volume={56},
  pages={9-16}
}
Genetic transformation of filamentous fungi is an essential tool in many areas such as biotechnology, medicine, phytopathology and genetics. However, available protocols to transform fungi are inefficient, laborious and have low reproducibility. We report the use of underwater shock waves as a novel method to transform filamentous fungi. An experimental piezoelectric shock wave generator was designed to expose fungal conidia to heterologous DNA. The device was successfully tested in Aspergillus… CONTINUE READING