A novel and automated assay for thiol/disulphide homeostasis.

@article{Erel2014ANA,
  title={A novel and automated assay for thiol/disulphide homeostasis.},
  author={Ozcan Erel and Salim Neşelioğlu},
  journal={Clinical biochemistry},
  year={2014},
  volume={47 18},
  pages={
          326-32
        }
}
View on PubMed
ybu.edu.tr
620 Citations
Highly Influential Citations
64
Background Citations
193
Methods Citations
257
Results Citations
10

Figures from this paper

620 Citations

Is disulphide/thiol ratio related to blood pressure in masked hypertension?
TLDR
It is found that dynamic thiol/disulphide homeostasis shifted towards disulphides formation due to thiol oxidation in patients with MHT.
Evaluation of the level of dynamic thiol/disulphide homeostasis in adolescent patients with newly diagnosed primary hypertension
TLDR
The study showed that dynamic thiol/disulphide homeostasis shifted towards disulphides formation in adolescent patients with primary HT, and might provide new therapeutic intervention strategies for patients.
Dynamic thiol/disulphide homeostasis in patients with newly diagnosed primary hypertension.
ABTS radical-based single reagent assay for simultaneous determination of biologically important thiols and disulfides.
Dynamic Thiol-Disulphide Homeostasis in Patients with Multiple Sclerosis
TLDR
Thiol-disulphide balance was shifted to the oxidative area during the relapse and these homeostasis parameters, which can be easily measured by this newly developed method at low costs, may be important in terms of showing the disease activity and presenting the underlying mechanisms of the disease.
Thiol-disulfide homeostasis: an integrated approach with biochemical and clinical aspects
TLDR
A growing body of evidence has demonstrated that the thiol-disulfide homeostasis is involved in variety diseases, such as diabetes mellitus, hypertension, non-small cell lung cancer, Familial Mediterranean fever, inflammatory bowel diseases, occupational diseases, gestational Diabetes mellitus and preeclampsia.
Thiol-disulphide Homeostasis in Essential Thrombocythemia Patients
TLDR
The study results showed that thiol-disulphide homeostasis shifted to the proliferative side in ET, in which ineffective erythropoiesis was predominant, which suggests that thrombotic complications may be reduced if the formation is achieved of mechanisms that will trigger the increase of disulphid groups.
Investigation of dynamic thiol–disulphide homoeostasis in age-related cataract patients with a novel and automated assay
TLDR
The study showed that dynamic thiol–disulphide homoeostasis has shifted towards disulphides formation, as a result of thiol oxidation in ARC patients, which supports the hypothesis that cataract is an oxidative disorder.
Evaluation of dynamic serum thiol-disulphide homeostasis in colorectal cancer
Dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis
TLDR
The totalThiol, native thiol, and disulfide levels in DKA decrease in favor of oxidative stress.
...
...

References

SHOWING 1-10 OF 26 REFERENCES
Plasma thiols redox status by laser‐induced fluorescence capillary electrophoresis
TLDR
An improvement of the previous method that allows the measurement of different thiol forms is presented, and the assay was used to measure the thiols redox status in 15 plasma samples from healthy volunteers.
The thiol pool in human plasma: the central contribution of albumin to redox processes.
Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid.
Quantification of thiols and disulfides.
Measurement of oxidized glutathione by enzymatic recycling coupled to bioluminescent detection.
TLDR
The sensitivity of this method is at the picomole level and is convenient for determination of G SSG physiological concentrations in tissues: GSSG levels measured in rat liver and kidney ranged from 76 to 215 and 52 to 170 nmol/g wet weight, respectively.
A precise method for the determination of whole blood and plasma sulfhydryl groups.
Spectrophotometric measurement of mercaptans with 4,4'-dithiodipyridine.
TLDR
4,4'-dithiodipyridine (DTDP) was found to quantitatively react with nonpolar thiols when triethylamine was used as catalyst, intense light absorption was seen when the reaction was terminated with acetic acid, and the spectrophotometric responses were independent of the nonthiol portions of the mercaptans.
Plasma Cysteine/Cystine Reduction Potential Correlates with Plasma Creatinine Levels in Chronic Kidney Disease
TLDR
Plasma Eh(Cyss/2Cys) correlated with increased levels of plasma creatinine, supporting the view that uremia triggers oxidative stress and may be used as a quantitative oxidative stress biomarker in uremic conditions.
Glutathione and thioredoxin redox during differentiation in human colon epithelial (Caco-2) cells.
TLDR
The aim of this study was to determine the redox state of cellular GSH/glutathione disulfide (GSH/GSSG) and Trx as a consequence of progression from proliferation to contact inhibition and spontaneous differentiation in colon carcinoma (Caco-2) cells.
Quantitation of reduced glutathione and cysteine in human immunodeficiency virus‐infected patients
TLDR
Capillary zone electrophoresis‐based assays investigated the relationship between plasma and intracellular thiol levels and HIV‐1 viremia in plasma and found that a decrease in GSH and Cys levels may be associated with disease progress.
...
...