A novel alpha-amylase gene is transiently upregulated during low temperature exposure in apple fruit.

@article{Wegrzyn2000ANA,
  title={A novel alpha-amylase gene is transiently upregulated during low temperature exposure in apple fruit.},
  author={Teresa F. Wegrzyn and Kim Reilly and Guido Cipriani and Paula Murphy and Robert Newcomb and Rebecca Gardner and Elspeth A Macrae},
  journal={European journal of biochemistry},
  year={2000},
  volume={267 5},
  pages={1313-22}
}
An alpha-amylase gene product was isolated from apple fruit by reverse-transcriptase PCR using redundant primers, followed by 5' and 3' RACE. The gene is a member of a small gene family. It encodes a putative 46.9 kDa protein that is most similar to an alpha-amylase gene from potato (GenBank accession M79328). In apple fruit this new gene was expressed at low levels, as detected by reverse-transcriptase PCR, in a number of plant tissues and during fruit development. Highest levels of mRNA for… CONTINUE READING

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