A novel alliinase from onion roots. Biochemical characterization and cDNA cloning.

@article{Lancaster2000ANA,
  title={A novel alliinase from onion roots. Biochemical characterization and cDNA cloning.},
  author={J. E. Lancaster and M. L. Shaw and M. Joyce and J. McCallum and M T McManus},
  journal={Plant physiology},
  year={2000},
  volume={122 4},
  pages={
          1269-79
        }
}
  • J. E. Lancaster, M. L. Shaw, +2 authors M T McManus
  • Published 2000
  • Biology, Medicine
  • Plant physiology
  • We have purified a novel alliinase (EC 4.4.1.4) from roots of onion (Allium cepa L.). Two isoforms with alliinase activity (I and II) were separated by concanavalin A-Sepharose and had molecular masses of 52.7 (I) and 50.5 (II) kD on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 51 (I) and 57.5 (II) kD by gel filtration fast-protein liquid chromatography. Isoform I had an isoelectric point of 9.3, while isoform II had isoelectric points of 7.6, 7.9, 8.1, and 8.3. The isoforms… CONTINUE READING
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    References

    SHOWING 1-10 OF 41 REFERENCES
    Alliin Lyase (Alliinase) from Garlic (Allium sativum)
    • 83
    Alliin lyase: preparation and characterization of the homogeneous enzyme from onion bulbs.
    • 35
    Purification of the alliin lyase of garlic, Allium sativum L.
    • 65
    Purification and Characterization of a C-S-Lyase from Ramson, the Wild Garlic,Allium ursinum.
    • 22
    On the Isolation and Characterization of a C-S-Lyase Preparation from Leek,Allium porrum.
    • 16
    Characterization of alliinase of Allium cepa (onion).
    • 73
    • Highly Influential
    Purification and properties of S-alkyl-L-cysteine lyase from seedlings of Acacia farnesiana Willd.
    • 42
    Purification and characterization of S-alkylcysteine alpha,beta-lyase from Pseudomonas putida.
    • 2