A novel TWO-STEP renaturation procedure for efficient production of recombinant BMP-2.

@article{Einem2010ANT,
  title={A novel TWO-STEP renaturation procedure for efficient production of recombinant BMP-2.},
  author={Sabrina von Einem and Elie Schwarz and Robert Rudolph},
  journal={Protein expression and purification},
  year={2010},
  volume={73 1},
  pages={65-9}
}
Bone morphogenetic proteins (BMPs) stimulate bone formation and thus constitute important protein therapeutics. Here, a novel procedure is presented which allows fast and efficient production of biologically active BMP-2 via a TWO-STEP procedure: the conditions are designed such that the first step favors formation of monomeric species with the correct intramolecular disulfide bridges, the conditions of the second folding reaction stimulate the formation of the intermolecular disulfide bridge… CONTINUE READING

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