A novel Sp1-related cis element involved in intestinal alkaline phosphatase gene transcription.

@article{Kim1999ANS,
  title={A novel Sp1-related cis element involved in intestinal alkaline phosphatase gene transcription.},
  author={J H Kim and Shufen Meng and Amy Shei and Richard Aaron Hodin},
  journal={The American journal of physiology},
  year={1999},
  volume={276 4 Pt 1},
  pages={G800-7}
}
We have used sodium butyrate-treated HT-29 cells as an in vitro model system to study the molecular mechanisms underlying intestinal alkaline phosphatase (IAP) gene activation. Transient transfection assays using human IAP-CAT reporter genes along with DNase I footprinting were used to localize a critical cis element (IF-III) corresponding to the sequence 5'-GACTGGGCGGGGTCAAGATGGA-3'. Deletion of the IF-III element resulted in a dramatic reduction in reporter gene activity, and IF-III was shown… CONTINUE READING

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