A novel SP-1 site in the human interleukin-1 beta promoter confers preferential transcriptional activity in keratinocytes.

@article{Husmann1996ANS,
  title={A novel SP-1 site in the human interleukin-1 beta promoter confers preferential transcriptional activity in keratinocytes.},
  author={Matthias Husmann and P Jehnichen and Bernhard Jahn and Dominik Schlosshan and E Romahn and Jean Marx},
  journal={European journal of immunology},
  year={1996},
  volume={26 12},
  pages={3008-14}
}
To investigate the mechanisms of transcriptional activation of interleukin-1beta (IL-1beta) in non-monocytic cells, we constructed a series of reporter plasmids with the bacterial chloramphenicol acetyltransferase gene linked to various parts of the human IL-1beta promoter and performed transient transfection experiments. We identified a promoter segment that activates transcription most efficiently in keratinocytes. Electrophoretic mobility shift assays (EMSA) with a 43-mer oligonucleotide… CONTINUE READING

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