A novel N-linked flagellar glycan from Methanococcus maripaludis.

  title={A novel N-linked flagellar glycan from Methanococcus maripaludis.},
  author={John F Kelly and Susan M Logan and Ken F Jarrell and David Vandyke and Evgeny V Vinogradov},
  journal={Carbohydrate research},
  volume={344 5},
The archaea Methanococcus maripaludis strain Mm900 produces flagella that are glycosylated with an N-linked tetrasaccharide. Mass spectrometric analysis of flagellar tryptic peptides identified a number of tryptic glycopeptides carrying a glycan of mass 1036.4Da, and fragmentation of the glycan oxonium ion indicated that the glycan was a tetrasaccharide. The glycan was purified, following extensive pronase digestion of flagellar filaments, by size-exclusion and anion-exchange chromatography… CONTINUE READING


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