A novel EBNA-1 tag system for high level expression and efficient detection of fusion proteins in vitro and in vivo.

@article{Chen2000ANE,
  title={A novel EBNA-1 tag system for high level expression and efficient detection of fusion proteins in vitro and in vivo.},
  author={M R Chen and Hester Huang and Chen Fen and Jin Yun Helen Chen},
  journal={Journal of virological methods},
  year={2000},
  volume={85 1-2},
  pages={35-41}
}
A highly specific monoclonal antibody, which recognises an epitope between amino acids (aa) 408 and 446 of EBNA-1, was found to immunoprecipitate this protein with great efficiency. By adding a 39-aa tag, the BGLF4 protein product of EBV was shown to express in the cytoplasm of transfected cells. This EBNA-1 tag could be used for the detection of specific protein expression by immunoblotting, immunofluorescence and, especially, immunoprecipitation assays. A plasmid vector encoding this EBNA-1… CONTINUE READING

Citations

Publications citing this paper.

Similar Papers

Loading similar papers…