A novel α-galactosidase from Βifidobacterium bifidum with transgalactosylating properties: gene molecular cloning and heterologous expression

@article{Goulas2008AN,
  title={A novel α-galactosidase from Βifidobacterium bifidum with transgalactosylating properties: gene molecular cloning and heterologous expression},
  author={Theodoros K. Goulas and Athanasios K. Goulas and George Tzortzis and Glenn R M Gibson},
  journal={Applied Microbiology and Biotechnology},
  year={2008},
  volume={82},
  pages={471-477}
}
A genomic library of Bifidobacterium bifidum (NCIMB 41171) DNA was constructed in Escherichia coli RA11r (melA−B+) and one α-galactosidase encoding gene was isolated. Conceptual translation combined with insertional mutagenesis analysis indicated an open reading frame (ORF) of 759 amino acid (aa) residues encoding an α-galactosidase (named as MelA) of 82.8 kDa. Partial purification and characterisation showed that the enzyme had an apparent native molecular mass of ≈243 kDa and a subunit size… CONTINUE READING
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