A novel , single-tube enzymatic fragmentation and library construction method enables fast turnaround times and improved data quality for microbial whole-genome sequencing

@inproceedings{Miller2017AN,
  title={A novel , single-tube enzymatic fragmentation and library construction method enables fast turnaround times and improved data quality for microbial whole-genome sequencing},
  author={Bronwen J. Miller and Victoria van Kets},
  year={2017}
}
Next-generation whole genome sequencing of microbes demands rapid, robust, and scalable library construction workflows, capable of generating high-quality sequence data across a wide range of genome sizes, complexities and genomic GC content. In this Application Note, we describe a streamlined library preparation method that results in minimal bias, high uniform coverage, and facilitates de novo assembly of microbial genomes.