A novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12.

@article{Miki2008ANS,
  title={A novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12.},
  author={Takeyoshi Miki and Yoshihiro Yamamoto and Hideo Matsuda},
  journal={Methods in molecular biology},
  year={2008},
  volume={416},
  pages={
          195-204
        }
}
We developed a novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12. The basic idea of the method is to randomly disrupt the genes on the DNA fragments cloned on the Kohara library by inserting a mini-transposon first, and then transfer the disrupted genes from the lambda vector to the E. coli chromosome by homologous recombination. Using this method, we constructed a set of 8402 Km(r) cis-diploid mutants harboring a mini-Tn10… CONTINUE READING
BETA