A novel, highly efficient peptide-HLA class I binding assay using unfolded heavy chain molecules: identification of HIV-1 derived peptides that bind to HLA-A*0201 and HLA-A*0301.

@article{Tan1997ANH,
  title={A novel, highly efficient peptide-HLA class I binding assay using unfolded heavy chain molecules: identification of HIV-1 derived peptides that bind to HLA-A*0201 and HLA-A*0301.},
  author={Tong Ling Tan and Annemieke Geluk and Mireille Toebes and Tom Henricus Maria Ottenhoff and Jan Wouter Drijfhout},
  journal={Journal of immunological methods},
  year={1997},
  volume={205 2},
  pages={
          201-9
        }
}
A novel cell-free, highly automated peptide-HLA binding assay has been designed during which a mixture of unfolded recombinant HLA heavy chain molecules, beta 2-microglobulin and a fluorescent labeled standard peptide is allowed to form peptide-HLA complexes. The binding of a peptide of interest is monitored as the ability to inhibit the formation of fluorescent peptide-HLA complexes. The assay was validated using published, known HLA-A* 0201 and HLA-A* 0301 binding peptides. In addition a… CONTINUE READING
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