A note on the identity of porcine liver carboxylesterase and prolyl-beta-naphthylamidase.

Abstract

Prolyl-beta-naphthylamidase from porcine liver is compared with the two prevalent isoenzymes of pig liver carboxylesterase by isoelectrofocusing experiments and by inhibition studies with phenyl-methyl-sulfonyl fluoride. The results suggest that prolyl-beta-naphthylamidase is identical with the amide-cleaving isoenzyme of carboxylesterase, not with the usually predominant methyl butyrate-hydrolysing isoenzyme. It is questionable whether the recently published sequence of prolyl-beta-naphthylamidase does belong to this enzyme or to the predominant carboxylesterase without amidase activity. Surprisingly, the amide-cleaving carboxylesterase isoenzymes from rat liver have almost no activity with prolyl-beta-naphthylamide.

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@article{Heymann1993ANO, title={A note on the identity of porcine liver carboxylesterase and prolyl-beta-naphthylamidase.}, author={Eckward W. Heymann and Krisztina Peter}, journal={Biological chemistry Hoppe-Seyler}, year={1993}, volume={374 11}, pages={1033-6} }