A nonviral minicircle vector for deriving human iPS cells

  title={A nonviral minicircle vector for deriving human iPS cells},
  author={Fangjun Jia and Kitchener D. Wilson and Ning Sun and Deepak M Gupta and Mei Huang and Zongjin Li and Nicholas J Panetta and Zhi Ying Chen and Robert Clayton Robbins and Mark A. Kay and Michael Thornton Longaker and Joseph C Wu},
  journal={Nature Methods},
Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells. 
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