Corpus ID: 56226671

A nonsense mutation in the porcine myostatin gene is a candidate causative variant for a recessive leg weakness syndrome and affects muscle depth

  title={A nonsense mutation in the porcine myostatin gene is a candidate causative variant for a recessive leg weakness syndrome and affects muscle depth},
  author={O. Matika and D. Robledo and R. Pong-Wong and J. Woolliams and S. Bishop and V. Riggio and A E. and Hoste and G. Walling and A. Archibald and R. Houston},
Lameness in piglets is a major animal welfare and economic problem in pig production. Following observation of a high incidence of a leg weakness syndrome in a commercial pig lines, a variance components approach was used to assess the genetic basis of the condition. The results suggested a monogenic recessive mode of inheritance, and homozygosity mapping was used to identify a region associated with the leg weakness syndrome on SSC15. Whole genome resequencing of cases and controls identified… Expand


Double muscling in cattle due to mutations in the myostatin gene.
  • A. Mcpherron, S. J. Lee
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1997
The similarity in phenotypes of double-muscled cattle and myostatin null mice suggests that mystatin performs the same biological function in these two species and is a potentially useful target for genetic manipulation in other farm animals. Expand
Generation of cloned adult muscular pigs with myostatin gene mutation by genetic engineering
These MSTN-KO boars grew normally to adulthood and showed visually-clear hypermuscular characteristics, increased carcass dressing percentage and loin eye size, and decreased backfat thickness, suggesting these pigs may show greater meat production, as well as being used in animal models of human diseases. Expand
Efficient Generation of Myostatin Mutations in Pigs Using the CRISPR/Cas9 System
The gene editing platform used in this study can efficiently generate genetically modified pigs with biological safety and demonstrates that Cas9/gRNA can induce non-homologous end-joining, long fragment deletions/inversions and homology-directed repair at the MSTN locus of PFFs. Expand
Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets
It is demonstrated that loss of MSTN increases muscle mass in pigs, which may help increase pork production for consumption in the future. Expand
Highly effective SNP-based association mapping and management of recessive defects in livestock
It is shown that the availability of genome-wide, high-density SNP panels, combined with the typical structure of livestock populations, markedly accelerates the positional identification of genes and mutations that cause inherited defects. Expand
Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP
Precision editing of pig MSTN gene has generated isozygous, SMG-free M STN KO cloned founders, which guaranteed a reliable route for elite livestock production and a strategy to minimize potential biological risks. Expand
Multiplex gene editing via CRISPR/Cas9 exhibits desirable muscle hypertrophy without detectable off-target effects in sheep
The findings suggested that the CRISPR/Cas9 method can be exploited as a powerful tool for livestock improvement by simultaneously targeting multiple genes that are responsible for economically significant traits. Expand
Analyses of pig genomes provide insight into porcine demography and evolution
The assembly and analysis of the genome sequence of a female domestic Duroc pig and a comparison with the genomes of wild and domestic pigs from Europe and Asia reveal a deep phylogenetic split between European and Asian wild boars ∼1 million years ago. Expand
Genome edited sheep and cattle
Zygote injection of TALEN mRNA can also produce gene-edited cattle and sheep and provides a practical alternative to somatic cell nuclear transfer for gene knockout or introgression of desirable alleles into a target breed/genetic line. Expand
Genetics of leg weakness in boars at the Danish pig breeding stations.
It was found that a long body predisposes the animal to leg weakness, whereas the correlations between daily gain and leg weakness characters in some cases were favourable. Expand