A nonradioactive DNA methyltransferase assay adaptable to high-throughput screening.

@article{Woo2005AND,
  title={A nonradioactive DNA methyltransferase assay adaptable to high-throughput screening.},
  author={Youn-Hi Woo and P T Ravi Rajagopalan and Stephen J Benkovic},
  journal={Analytical biochemistry},
  year={2005},
  volume={340 2},
  pages={336-40}
}
We have developed a nonradioactive assay method for DNA methyltransferases based on the ability to protect substrate DNA from restriction. DNA immobilized to a microplate well was treated sequentially with methyltransferase and an appropriate endonuclease. The amount of methylated DNA product is reflected by a proportional decrease in endonuclease cleavage, which is in turn reflected by increased retention of the end-labeled affinity probe. A single universal substrate was designed to assay… CONTINUE READING