A nonisotopic assay for unambiguous assignment of DNA glycosylase functionality.

  • Heiko Kuhn
  • Published 2007 in Analytical biochemistry

Abstract

DNA glycosylase assays still commonly involve the use of radioactively end-labeled DNA substrates, although in recent years a few nonradioactive methods have been described [1–3]. All reported assays, however, have in common that they are strand cleavage assays. In contrast to DNA glycosylases with inherent strand-scission activity (i.e., DNA glycosylases… (More)

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