A non-radioactive DNA diagnostic procedure for the detection of malarial infection: general application to genome with repetitive sequences.

Abstract

A novel non-radioactive DNA diagnostic method has been developed to detect Plasmodium falciparum infection in whole blood. In this method a drop of blood from a finger prick is added to a lysing solution containing a biotinylated oligonucleotide whose sequence design is based on the repeated sequence of the parasite genome. The mixture is heated in a… (More)

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