A non-equilibrium isoelectric focusing method to determine states of phosphorylation of cardiac troponin I: identification of Ser-23 and Ser-24 as significant sites of phosphorylation by protein kinase C.

@article{Kobayashi2005ANI,
  title={A non-equilibrium isoelectric focusing method to determine states of phosphorylation of cardiac troponin I: identification of Ser-23 and Ser-24 as significant sites of phosphorylation by protein kinase C.},
  author={Tomoyoshi Kobayashi and Xiao-Feng Yang and Lori A Walker and Richard B van Breemen and R. John Solaro},
  journal={Journal of molecular and cellular cardiology},
  year={2005},
  volume={38 1},
  pages={213-8}
}
Phosphorylation of cardiac troponin I (cTnI) by cAMP-dependent kinase (PKA), protein kinase C (PKC) and potentially other kinases modulates the activity of myofilaments. To elucidate the signaling mechanisms involving this modulation, it is important to determine the phosphorylation states of cTnI and its phosphorylation sites in a simple and efficient… CONTINUE READING