A non-Golgi alpha 1,2-fucosyltransferase that modifies Skp1 in the cytoplasm of Dictyostelium.

@article{Wel2001ANA,
  title={A non-Golgi alpha 1,2-fucosyltransferase that modifies Skp1 in the cytoplasm of Dictyostelium.},
  author={Hanke van der Wel and Howard R. Morris and Maria Panico and Thanai Paxton and Simon J. North and Anne Dell and Jodi M. Thomson and Christopher M West},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 36},
  pages={33952-63}
}
Skp1 is a subunit of the SCF-E3 ubiquitin ligase that targets cell cycle and other regulatory factors for degradation. In Dictyostelium, Skp1 is modified by a pentasaccharide containing the type 1 blood group H trisaccharide at its core. To address how the third sugar, fucose alpha1,2-linked to galactose, is attached, a proteomics strategy was applied to determine the primary structure of FT85, previously shown to copurify with the GDP-Fuc:Skp1 alpha 1,2-fucosyltransferase. Tryptic-generated… CONTINUE READING