A nitrilotriacetic acid monooxygenase with conditional NADH-oxidase activity.

  title={A nitrilotriacetic acid monooxygenase with conditional NADH-oxidase activity.},
  author={Mary K. Firestone and Steven D. Aust and James M. Tiedje},
  journal={Archives of biochemistry and biophysics},
  volume={190 2},
Abstract A tertiary amine monoxygenase from a Pseudomonas sp. was partially purified (35-fold) and characterized. In the presence of nitrilotriacetate (NTA), O2, NADH, and Mn2+, the enzyme yielded two sets of products: iminodiacetate, glyoxylate, NAD+ and H2O; or H2O2 and NAD+. Which set of products predominated was a function of enzyme concentration, ionic strength of solution, pH, and cation supplied. NTA functioned both as a modifiable substrate and as a stimulator of NADH oxidase activity… 
Purification and characterization of a two-component monooxygenase that hydroxylates nitrilotriacetate from "Chelatobacter" strain ATCC 29600
An assay based on the consumption of nitrilotriacetate (NTA) was developed to measure the activity of NTA monooxygenase (NTA-Mo) in cell extracts of "Chelatobacter" strain ATCC 29600 and to purify a
Cloning and characterization of the genes encoding nitrilotriacetate monooxygenase of Chelatobacter heintzii ATCC 29600
A 6.2-kb DNA fragment containing the genes for the nitrilotriacetate (NTA) monooxygenase of Chelatobacter heintzii ATCC 29600 was cloned and characterized by DNA sequencing and expression studies, and the deduced gene product showed only significant homology to SoxA and SnaA and shared weak homologies in one domain with other NADH:flavine mononucleotide oxidoreductases.
Biochemistry and physiology of the degradation of nitrilotriacetic acid and other metal complexing agents
Synthetic organic, metal-sequestering compounds, such as the aminopoly-carboxylic acids (mainly EDTA and NTA) or the organophosphonic acids HEDP or ATMP, are included in a wide range of different
Microbial degradation of chelating agents used in detergents with special reference to nitrilotriacetic acid (NTA)
The physiological, biochemical and ecological aspects of the microbial degradation of the complexing agents most frequently used, such as polyphosphates, aminopolycarboxylates (especially of nitrilotriacetic acid), and phosphonates are reviewed.
Ion chromatography method for iminodiacetic acid determination in biological matrices in the presence of nitrilotriacetic acid
Abstract A sensitive ion Chromatographic method for the determination of iminodiacetic acid in the presence of nitrilotriacetic acid in cell-free extracts is described using a mixture of carbonate,
Effect of temperature and dissolved oxygen on biodegradation of nitrilotriacetate
Abstract The effect of temperature and dissolved oxygen on the rate of biodegradation of nitrilotriacetate (NTA) was examined in water samples collected from the Rur River. Biodegradation of NTA was
Determination of nitrilotriacetate in biological matrices using ion exclusion chromatography.
A sensitive method for the determination of nitrilotriacetate in biological growth media and cell-free extracts by ion exclusion chromatography is described using HCl as an eluant using a membrane suppressor and a conductivity detector.
A review of the environmental and mammalian toxicology of nitrilotriacetic acid.
The ability of NTA to chelate metal ions such as Mg++ and Ca++ into water soluble complexes makes NTA useful as an additive to boiler water, as a builder in laundry detergents, and as a stabilizer in textile, paper, and pulp processing.
Effects of dissolved oxygen concentration on biodegradation of 2,4-dichlorophenoxyacetic acid
Results suggest that dissolved oxygen concentrations below 1 mg/liter may be rate limiting for the biodegradation of chlorinated aromatic compounds such as 2,4-D, which have a requirement for molecular oxygen as a cosubstrate for metabolism.
The behaviour of nitrilotriacetic acid during the anaerobic digestion of co-settled sewage sludge
Abstract The removal of nitrilotriacetic acid (NTA) in four laboratory scale anaerobic digesters treating mixed primary sludge has been investigated. Two digesters received mixed primary sludge


Metabolism of nitrilotriacetate by cells of Pseudomonas species.
A Pseudomonas species was isolated from soil which could degrade nitrilotriacetate (NTA) to CO(2), H(2)O, NH(3), and cellular constituents without the accumulation of significant quantities of
Pathway of degradation of nitrilotriacetate by a Pseudomonas species
The pathway of degradation of nitrilotriacetate (NTA) was determined by using cell-free extracts and a 35-fold purification of NTA monooxygenase and an unstable alpha-hydroxy-NTA intermediate was postulated.
The metabolism of nitrilotriacetate by a pseudomonad.
Compared with growth on glucose, growth on nitrilotriacetate results in increased activities of enzymes of glycine and serine metabolism, namely serine hydroxymethyltransferase, glycine decarboxylase, serine-oxaloacetate aminotransferase and hydroxypyruvate reductase.
Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans.
An observation suggests that, during its shift from C-1 to C-2 of the nucleus, the side chain of the substrate remains bound to a site on the enzyme while a conformational change of the protein permits the necessary movement of the benzene ring.
Studies of a flavoprotein, salicylate hydroxylse. I. Enzyme mechanism.
The mechnism of action of this enzyme has been examined by spectrophotometric and stopped flow techniques, and the presence of salicylate facilitates DPNH binding and determines the rate of enzyme reduction to E-FADH2.
On the interaction of para‐hydroxybenzoate hydroxylase from Pseudomonas fluorescens with halogen ions
Inducible p-hydroxybenzoate hydroxylases from Pseudomonas species have been studied in detail by several groups [l-3]. During our investigations of p-hydroxybenzoate hydroxylase from Ps. fluorescens
Degradation of allantoin by Pseudomonas acidovorans.
Summary 1. The degradation of allantoin by Pseudomonas acidovorans was investigated. Allantoate, ureidoglycine, ureidoglycolate, glyoxylate and urea are considered to be intermediates in this
4 Flavin and Pteridine Monooxygenases
Publisher Summary The reactivity with oxygen of reduced flavins and flavoenzymes is one of the most interesting problems in the field of flavin chemistry. In the case of many flavoproteins, which are
The formation of an N-hydroxymethyl intermediate in the N-demethylation of N-methylcarbazole in vivo and in vitro.
  • J. Gorrod, D. Temple
  • Biology, Medicine
    Xenobiotica; the fate of foreign compounds in biological systems
  • 1976
N-Hydroxymethylcarbazole decomposed spontaneously in solution at pH 7-4 to formaldehyde and carbazole; this breakdown was enhanced by incubation with liver microsomes from several species, especially the guinea-pig.
Protein measurement with the Folin phenol reagent.
Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.