A new yeast gene with a myosin-like heptad repeat structure


We isolated a gene encoding a 218 kDa myosin-like protein from Saccharomyces cerevisiae using a monoclonal antibody directed against human platelet myosin as a probe. The protein sequence encoded by the MLP1 gene (for myosin-like protein) contains extensive stretches of a heptad-repeat pattern suggesting that the protein can form coiled coils typical of myosins. Immunolocalization experiments using affinity-purified antibodies raised against a TrpE-MLP1 fusion protein showed a dot-like structure adjacent to the nucleus in yeast cells bearing the MLP1 gene on a multicopy plasmid. In mouse epithelial cells the yeast anti-MLP1 antibodies stained the nucleus. Mutants bearing disruptions of the MLP1 gene were viable, but more sensitive to ultraviolet light than wild-type strains, suggesting an involvement of MLP1 in DNA repair. The MLP1 gene was mapped to chromosome 11, 25 cM from met1.

DOI: 10.1007/BF00279439

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@article{Koelling1993ANY, title={A new yeast gene with a myosin-like heptad repeat structure}, author={R . A . Koelling and Thu H.M. Nguyen and Ellson Y. Chen and David Botstein}, journal={Molecular and General Genetics MGG}, year={1993}, volume={237}, pages={359-369} }