A new way to rapidly create functional, fluorescent fusion proteins: random insertion of GFP with an in vitro transposition reaction

@article{Sheridan2002ANW,
  title={A new way to rapidly create functional, fluorescent fusion proteins: random insertion of GFP with an in vitro transposition reaction},
  author={Douglas L Sheridan and Catherine H. Berlot and Antoine Robert and Fiona M. Inglis and Klara B Jakobsd{\'o}ttir and James R. Howe and Thomas E Hughes},
  journal={BMC Neuroscience},
  year={2002},
  volume={3},
  pages={7 - 7}
}
The jellyfish green fluorescent protein (GFP) can be inserted into the middle of another protein to produce a functional, fluorescent fusion protein. Finding permissive sites for insertion, however, can be difficult. Here we describe a transposon-based approach for rapidly creating libraries of GFP fusion proteins. We tested our approach on the glutamate receptor subunit, GluR1, and the G protein subunit, αs. All of the in-frame GFP insertions produced a fluorescent protein, consistent with the… CONTINUE READING

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