A new vector, based on the PolII promoter of the U1 snRNA gene, for the expression of siRNAs in mammalian cells.

@article{Denti2004ANV,
  title={A new vector, based on the PolII promoter of the U1 snRNA gene, for the expression of siRNAs in mammalian cells.},
  author={Michela Denti and Alessandro Rosa and Olga Sthandier and Fernanda Gabriella De Angelis and Irene Bozzoni},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2004},
  volume={10 1},
  pages={
          191-9
        }
}
  • Michela Denti, Alessandro Rosa, +2 authors Irene Bozzoni
  • Published in
    Molecular therapy : the…
    2004
  • Biology, Medicine
  • Several vectors for the induction of RNA interference in mammalian cells have been described,based mainly on polIII-dependent promoters. They transcribe short hairpin RNAs (shRNA) that,after being processed into short interfering RNAs (siRNAs), mediate the degradation of the target mRNA. Here, we describe the construction of a new siRNA-expressing vector (psiUx) based on the strong and ubiquitous polII-dependent promoter of the human U1 small nuclear RNA (snRNA)gene. In psiUx, the only… CONTINUE READING

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