A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer.

@article{Boshart1984ANT,
  title={A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer.},
  author={Michael Boshart and Lutz Prof Dr Gissmann and Hans Ikenberg and Andreas Kleinheinz and Wolfram Scheurlen and Harald Hausen},
  journal={The EMBO Journal},
  year={1984},
  volume={3}
}
DNA of a new papillomavirus type was cloned from a cervical carcinoma biopsy. Two EcoRI clones of 7.8 and 6.9 kb in length were obtained, the latter contained a 900‐bp deletion. The BamHI fragments of both clones were used to characterize the DNA. It represents a distinct type of papillomavirus as determined by its size, its cross‐hybridization with DNA of other papillomavirus types under conditions of low stringency only, the co‐linear alignment of its genome with HPV 6 and HPV 16 prototypes… 
Human papillomavirus (hpv) DNA in penile carcinomas and in two cell lines from high‐incidence areas for genital cancers in Africa
TLDR
To isolate and characterize a possible highly oncogenic HPV, a genomic library of the DNA extracted from the PC‐8 biopsy has been constructed in the EcoRI arms of the EMBL4 phage.
Human papillomavirus type 16 DNA from a vulvar carcinoma in situ is present as head-to-tail dimeric episomes with a deletion in the non-coding region.
TLDR
A number of genital cancer biopsy samples were screened for the presence of human papillomavirus type 16 (HPV-16) DNA sequences and a deletion within the non-coding region, a region which includes the viral origin of DNA replication and transcriptional control sequences was found.
Presence of human papillomavirus type 18 DNA in vulvar carcinomas and its integration into the cell genome.
TLDR
In these tumours the integration of HPV-18 DNA seems to occur in the E1/E2 region of the virus genome with long deletions in almost all samples, suggesting that the integration event and the possible expression of these sequences could play a role in some stages of cellular transformation.
Detection of human papillomavirus type 16 DNA and evidence for integration into the cell DNA in cervical dysplasia.
The presence of human papillomavirus (HPV) type 16 DNA in biopsies from precancerous lesions and from early lesions of human cervical cancer, and the integration of virus DNA into host cell DNA were
A high frequency of human papillomavirus DNA sequences in cervical carcinomas of indian women as revealed by southern blot hybridization and polymerase chain reaction
TLDR
The findings suggest that infection with HPV is an important etiologic factor for the development of cervical cancer, that a number of such tumours may arise without HPV infection, and that integration of the viral DNA into host genome is not always essential for malignant progression.
Characterization of a new human papillomavirus (HPV 41) from disseminated warts and detection of its DNA in some skin carcinomas
TLDR
A new human papilla ma virus type (HPV 41), distantly related to known HPV prototypes, was isolated from a patient with disseminated facial, peri‐anal and foot warts and classification of this cloned DNA as a new type is based on the degree of cross‐hybridization with 40 HPV types under conditions of varying stringency.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 23 REFERENCES
Molecular Cloning: A Laboratory Manual
TLDR
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
Development in Molecular Virology
  • Development in Molecular Virology
  • 1984
Rev. Physiol. Biochem. Pharmacol
  • Rev. Physiol. Biochem. Pharmacol
  • 1984
Cancer Res
  • Cancer Res
  • 1983
Proc. NatI. Acad. Sci. USA
  • Proc. NatI. Acad. Sci. USA
  • 1983
Proc. Natl. Acad. Sci. USA
  • Proc. Natl. Acad. Sci. USA
  • 1983
J. Gen. Virol
  • J. Gen. Virol
  • 1982
J. Virol
  • J. Virol
  • 1982
Proc. NatI. Acad. Sci. USA
  • Proc. NatI. Acad. Sci. USA
  • 1982
Proc. Natl. Acad. Sci. USA
  • Proc. Natl. Acad. Sci. USA
  • 1982
...
1
2
3
...