A new troponin T and cDNA clones for 13 different muscle proteins, found by shotgun sequencing

@article{Putney1983ANT,
  title={A new troponin T and cDNA clones for 13 different muscle proteins, found by shotgun sequencing},
  author={Scott D. Putney and Walter C. Herlihy and Paul R. Schimmel},
  journal={Nature},
  year={1983},
  volume={302},
  pages={718-721}
}
Complete amino acid sequences have been established for 19 muscle-related proteins and these proteins are each sufficiently abundant to suggest that their mRNA levels are about 0.4% or higher. Based on these considerations, a simple theoretical analysis shows that clones for most of these proteins can be identified within a complementary DNA library by sequencing cDNA inserts from 150–200 randomly selected clones. This procedure should not only rigorously identify specific clones, but it could… 
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References

SHOWING 1-10 OF 36 REFERENCES
Troponin C from rabbit slow skeletal and cardiac muscle is the product of a single gene.
TLDR
Troponin C has been isolated from rabbit slow skeletal and cardiac muscle and the complete amino acid sequence of the slow muscle protein determined and it seems very likely that trop onin C from these two tissues is the product of a single gene, in contrast to troponin I and Troponin T which are the products of different genes.
cDNA clone analysis of six co-regulated mRNAs encoding skeletal muscle contractile proteins.
  • K. Hastings, C. Emerson
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1982
TLDR
Findings indicate that coordinate activation of contractile protein synthesis during myogenesis is controlled by mechanisms that direct the accumulation ofcontractile protein mRNAs rather than their translational utilization.
Amino-acid sequence of tropomyosin-binding component of rabbit skeletal muscle troponin.
TLDR
The complete amino-acid sequence of rabbit skeletal troponin-T is reported and predictions of secondary structure indicate 37% helical content with two long sections in that portion of the molecule implicated in binding to tropomyocin.
The amino-acid sequence of the alkali light chains of rabbit skeletal-muscle myosin.
TLDR
The amino-acid sequence of the alkali light chain 1 (Al) of rabbit skeletal muscle myosin has been determined and compared with the sequences of the smaller but related alkala light chain 2 (A2), and it was concluded that either A2 was a degraded fragment of A1 or the two proteins showed an exceptionally high degree of homology.
Molecular cloning of mRNA sequences for cardiac alpha- and beta-form myosin heavy chains: expression in ventricles of normal, hypothyroid, and thyrotoxic rabbits.
TLDR
It is concluded that pM HC alpha and pMHC beta contain sequences of the alpha- and beta-form myosin heavy chain genes, respectively.
The components of troponin from chicken fast skeletal muscle. A comparison of troponin T and troponin I from breast and leg muscle.
TLDR
A comparison of the troponin I and C components from breast and leg muscle by amino acid analysis, gel electrophoresis and peptide 'mapping' provides strong evidence for the identity of these proteins.
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