A new transgenic mouse mutagenesis test system using Spi- and 6-thioguanine selections.

@article{Nohmi1996ANT,
  title={A new transgenic mouse mutagenesis test system using Spi- and 6-thioguanine selections.},
  author={Takehiko Nohmi and Motonobu Katoh and Hiroshi Suzuki and Mitsuaki Matsui and Masami Yamada and Masahito Watanabe and Masakuni Suzuki and N. Horiya and Otoya Ueda and Toshio Shibuya and Haruhiko Ikeda and Toshio Sofuni},
  journal={Environmental and molecular mutagenesis},
  year={1996},
  volume={28 4},
  pages={
          465-70
        }
}
A new transgenic mouse mutagenesis test system has been developed for the efficient detection of point mutations and deletion mutations in vivo. The mice carry lambda EG10 DNA as a transgene. When the rescued phages are infected into Escherichia coli YG6020-expressing Cre recombinase, the phage DNA is converted into plasmid pYG142 carrying the chloramphenicol-resistance gene and the gpt gene of E. coli. The gpt mutants can be positively detected as colonies arising on plates containing… CONTINUE READING
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