A new transgene assay to study microsatellite instability in wild-type and mismatch-repair defective plant progenies

@inproceedings{Depeiges2005ANT,
  title={A new transgene assay to study microsatellite instability in wild-type and mismatch-repair defective plant progenies},
  author={Annie Depeiges and Stephane Farget and Fabienne Degroote and Georges Picard},
  year={2005}
}
Abstract DNA mismatch repair (MMR) genes are essential for maintaining the fidelity of DNA replication in prokaryotes and eukaryotes. In most species, from Escherichia coli to human, alterations in this system result in elevated frequencies of microsatellite length alterations. In the present report, we designed an in planta assay system to measure the frequencies of microsatellite instability in Arabidopsis thaliana . A transgenic reporter line was selected in which a (CT) 23 dinucleotide… CONTINUE READING