A new technique to expand human mesenchymal stem cells using basement membrane extracellular matrix.

@article{Matsubara2004ANT,
  title={A new technique to expand human mesenchymal stem cells using basement membrane extracellular matrix.},
  author={Takehiro Matsubara and Shinichi Tsutsumi and Haiou Pan and Hisatada Hiraoka and Ryo Oda and Masahiro Nishimura and Hiroshi Kawaguchi and Kouzou Nakamura and Yukio Kato},
  journal={Biochemical and biophysical research communications},
  year={2004},
  volume={313 3},
  pages={503-8}
}
Mesenchymal stem cells (MSC) show a very short proliferative life span and readily lose the differentiation potential in culture. However, the growth rate and the proliferative life span of the stem cells markedly increased using tissue culture dishes coated with a basement membrane-like extracellular matrix, which was produced by PYS-2 cells or primary endothelial cells. Furthermore, the stem cells expanded on the extracellular matrix, but not those on plastic tissue culture dishes, retained… CONTINUE READING