A new technique for assaying cytochrome P450 enzyme activity in a single cell

Abstract

A new microspectrofluorometric technique for measuring the ethoxyresorufin-O-deethylase (EROD) activity of cytochrome P450 (CYP)1A1 in single living cells is described. The system, which uses a perfusion chamber and an HPLC pump, allowed cells to be stained, fixed, blocked, and washed by injecting each treatment solution into the on-line carrier stream of… (More)
DOI: 10.1007/s10565-006-0055-7

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