A new subtilisin family: nucleotide and deduced amino acid sequences of new high-molecular-mass alkaline proteases from Bacillus spp.

@article{Okuda2004ANS,
  title={A new subtilisin family: nucleotide and deduced amino acid sequences of new high-molecular-mass alkaline proteases from Bacillus spp.},
  author={Mitsuyoshi Okuda and Nobuyuki Sumitomo and Yasushi Takimura and Akinori Ogawa and Katsuhisa Saeki and Shuji Kawai and Tohru Kobayashi and Susumu Ito},
  journal={Extremophiles},
  year={2004},
  volume={8},
  pages={229-235}
}
Six genes encoding high-molecular-mass subtilisins (HMSs) of alkaliphilic Bacillus spp. were cloned and sequenced. Their open reading frames of 2,394–2,424 bp encoded prosubtilisins of 798–808 amino acids (aa) consisting of the prepropeptides of 151–158 aa and the mature enzymes of 640–656 aa. The deduced aa sequences of the mature enzymes exhibited 60–95% identity to those of FT protease of Bacillus sp. strain KSM-KP43, a subtilisin-like serine protease, and a minor serine protease, Vpr, of… 
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