A new strategy in design of +RNA virus infectious clones enabling their stable propagation in E. coli.

  title={A new strategy in design of +RNA virus infectious clones enabling their stable propagation in E. coli.},
  author={Vladimir F Yamshchikov and Vasiliy P. Mishin and Fabio Cominelli},
  volume={281 2},
Infectious clone methodology is a valuable tool of modern experimental virology. However, its use is often constrained by the instability of infectious clone constructs during propagation in E. coli. To circumvent this problem, we have devised a strategy that could be suitable for design of +RNA virus molecular clones in general. An infectious clone is assembled as "infectious DNA," and expression of problem regions present in the viral cDNA is prevented during propagation in E. coli by… 

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