A new staining protocol for detection of murine antibody-secreting plasma cell subsets by flow cytometry.

Abstract

We provide a robust four-color fluorescence-based flow cytometry protocol that distinguishes viable dividing plasmablasts from nondividing plasma cells and, based on CD19 surface abundance, identifies two mature plasma cell populations in the spleen and the bone marrow of mice.

DOI: 10.1002/eji.201747019

Cite this paper

@article{Pracht2017ANS, title={A new staining protocol for detection of murine antibody-secreting plasma cell subsets by flow cytometry.}, author={Katharina Pracht and Julia Meinzinger and Patrick Daum and Sebastian R Schulz and Dorothea Reimer and Manuela Hauke and Edith Roth and Dirk Mielenz and Claudia Berek and Joana C{\^o}rte-Real and Michael L Atchison and Wolfgang Schuh}, journal={European journal of immunology}, year={2017}, volume={47 8}, pages={1389-1392} }