A new split‐luciferase complementation assay identifies pentachlorophenol as an inhibitor of apoptosome formation

@inproceedings{Tashakor2019ANS,
  title={A new split‐luciferase complementation assay identifies pentachlorophenol as an inhibitor of apoptosome formation},
  author={Amin Tashakor and Mahshid H‐Dehkordi and Enda O'Connell and Sergi Gomez Ganau and Rafael Gozalbes and Leif A. Eriksson and Saman Hosseinkhani and Howard O Fearnhead},
  booktitle={FEBS open bio},
  year={2019}
}
The expense and time required for in vivo reproductive and developmental toxicity studies have driven the development of in vitro alternatives. Here, we used a new in vitro split luciferase-based assay to screen a library of 177 toxicants for inhibitors of apoptosome formation. The apoptosome contains seven Apoptotic Protease-Activating Factor-1 (Apaf-1) molecules and induces cell death by activating caspase-9. Apaf-1-dependent caspase activation also plays an important role in CNS development… CONTINUE READING

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