A new specific antibody reveals octopamine‐like immunoreactivity in cockroach ventral nerve cord

  title={A new specific antibody reveals octopamine‐like immunoreactivity in cockroach ventral nerve cord},
  author={Manfred Eckert and J{\"u}rgen Rapus and Asja N{\"u}rnberger and Heinz Penzlin},
  journal={Journal of Comparative Neurology},
An antiserum was raised in rabbits immunized with octopamine conjugated to thyroglobulin. The specificity of this antiserum for octopamine is shown by dot blot immunoassay analysis. The antiserum does not crossreact with dopamine, noradrenaline, and serotonin, but slight crossreactivity with the amine tyramine at high concentrations was observed. The tyramine crossreactivity could be eliminated by preabsorption with a tyramine‐glutaraldehyde‐BSA conjugate. Using this antiserum, we describe the… 

Taurine-like immunoreactivity in octopaminergic neurones of the cockroach, Periplaneta americana (L.)

A comparison of the immunostaining for taurine and octopamine indicates that several of the taurin-like immunoreactive (-LI) neurones are probably members of theOctopamine-immunoreactive DUM cell population, and the possible physiological significance of this coexistence is discussed.

Tyramine as an independent transmitter and a precursor of octopamine in the locust central nervous system: An immunocytochemical study

It is concluded that tyramine is an independent transmitter in locusts, and that in octopaminergic neurons the ratio between octopamine and its precursor tyramsine is highly dynamic.

Distribution and developmental expression of octopamine‐immunoreactive neurons in the central nervous system of the leech

An antibody highly specific to octopamine was used to examine the distribution ofOctopamine‐immunoreactive neurons in the segmental ganglia of American and European medicinal leeches and determined the identity of the dorsolateral octopaminergic neurons.

Localization of corazonin in the nervous system of the cockroach Periplaneta americana

Results indicate that the production of highly specific antisera to some neuropeptides may require a considerable number of booster injections, and that the majority of this immunoreactivity was due to cross-reactivity.

Octopamine in the central nervous system of Oligochaeta: an immunocytochemical and biochemical study

OA appears to have a regulatory role in the central nervous system of Oligochaeta, and certain regions of the neuropil of the ganglia were densely innervated by immunoreactive fibers.

Octopamine-like immunoreactivity in the dorsal unpaired median (DUM) neurons innervating the accessory gland of the male cockroach Periplaneta americana

The combination of the cobalt-filling technique with immunohistochemical mapping of cells suggests an octopaminergic innervation of the musculature of the accessory gland by DUM neurons.

Cytoarchitecture of histamine‐, dopamine‐, serotonin‐ and octopamine‐containing neurons in the cricket ventral nerve cord

  • M. Hörner
  • Biology
    Microscopy research and technique
  • 1999
A comparative neuroanatomical description of the cellular localization of the biogenic amines histamine, dopamine, serotonin and octopamine in the ventral nerve cord of an insect, namely the cricket, Gryllus bimaculatus is provided.

Octopamine immunoreactivity in the fruit fly Drosophila melanogaster

Octopamine immunoreactivity in the adult CNS revealed many additional neurons compared to the larval CNS, indicating that at least a subset of adult octopamine neurons may differentiate during metamorphosis.



Specific Antisera Against the Catecholamines: L‐3,4‐Dihydroxyphenylalanine, Dopamine, Noradrenaline, and Octopamine Tested by an Enzyme‐Linked Immunosorbent Assay

The results suggest that the antibodies of L‐DOPA and DA antisera recognize preferentially the catechol moiety, whereas for the anti‐NA and anti‐OA antibodies, the lateral chain is important.

Distribution of GABA-like immunoreactivity in the central nervous system of the cockroach, Periplaneta americana (L.).

Identical distribution patterns of neuronal elements after immunofluorescence double-staining with anti-GABA antibodies and antibodies against the GABA-synthesizing enzyme glutamic acid decarboxylase demonstrated the high specificity of the anti- GABA serum used in this paper.

Analysis of Octopamine, Dopamine, 5-Hydroxytryptamine and Tryptophan in the Brain and Nerve Cord of the American Cockroach

The demonstrated importance of OA in insects, together with its potential significance in vertebrates, indicates the need for a convenient, sensitive procedure for simultaneous determination of Oa and other monoamines in small amounts of biological tissue.

A subpopulation of dorsal unpaired median neurons in the blood‐feeding insect Rhodnius prolixus displays serotonin‐like immunoreactivity

This work describes, for the first time in insects, the presence of serotonin‐like immunoreactive dorsal unpaired median (DUM) neurons and enhances the immunostaining of the cell bodies and axons of these DUM neurons by using 5,7‐dihydroxytryptamine and nerve transection.


  • P. Evans
  • Biology
    Journal of neurochemistry
  • 1978
Abstract— Octopamine distribution has been surveyed in the nervous systems of two insect species, the locust, Schistocerca americana gregaria, and the cockroach Periplaneta americana. It is

The brain-specific S-100 protein on neuronal cell membranes.

The S-100 protein has been localized to the neuronal plasma membranes of isolated Deiters' neurons by fluorescence microscopy using fluorescein-conjugated antiserum to the protein and by

Octopamine immunoreactive cell populations in the locust thoracic‐abdominal nervous system

It is suggested that probably all peripherally projecting DUM‐cells are octopaminergic in the examined ganglia and shown to label with an antiserum to γ‐amino butyric acid (GABA).

Octopamine in insects: neurotransmitter, neurohormone, and neuromodulator

It is shown that octopamine can act as a neurotransmitter, a neurohormone, and a neuromodulator within the nervous system of invertebrates and in locusts, an identified octopaminergic neuron innervates the extensor-tibiae muscle of the hind legs.

Effect of taurine on excitation-induced changes in brain and hemolymph octopamine levels in Periplaneta americana

The results indicate that taurine inhibits the release ofOctopamine from the insect nervous system and may form part of a dual control mechanism for excitation, with octopamine serving as a positive effector and taurines serving as an inhibitory modulator of octopamines release.