A new specific antibody reveals octopamine‐like immunoreactivity in cockroach ventral nerve cord

@article{Eckert1992ANS,
  title={A new specific antibody reveals octopamine‐like immunoreactivity in cockroach ventral nerve cord},
  author={Manfred Eckert and J{\"u}rgen Rapus and Asja N{\"u}rnberger and Heinz Penzlin},
  journal={Journal of Comparative Neurology},
  year={1992},
  volume={322}
}
An antiserum was raised in rabbits immunized with octopamine conjugated to thyroglobulin. The specificity of this antiserum for octopamine is shown by dot blot immunoassay analysis. The antiserum does not crossreact with dopamine, noradrenaline, and serotonin, but slight crossreactivity with the amine tyramine at high concentrations was observed. The tyramine crossreactivity could be eliminated by preabsorption with a tyramine‐glutaraldehyde‐BSA conjugate. Using this antiserum, we describe the… 

Taurine-like immunoreactivity in octopaminergic neurones of the cockroach, Periplaneta americana (L.)

A comparison of the immunostaining for taurine and octopamine indicates that several of the taurin-like immunoreactive (-LI) neurones are probably members of theOctopamine-immunoreactive DUM cell population, and the possible physiological significance of this coexistence is discussed.

Tyramine as an independent transmitter and a precursor of octopamine in the locust central nervous system: An immunocytochemical study

It is concluded that tyramine is an independent transmitter in locusts, and that in octopaminergic neurons the ratio between octopamine and its precursor tyramsine is highly dynamic.

Distribution and developmental expression of octopamine‐immunoreactive neurons in the central nervous system of the leech

An antibody highly specific to octopamine was used to examine the distribution ofOctopamine‐immunoreactive neurons in the segmental ganglia of American and European medicinal leeches and determined the identity of the dorsolateral octopaminergic neurons.

Localization of corazonin in the nervous system of the cockroach Periplaneta americana

Results indicate that the production of highly specific antisera to some neuropeptides may require a considerable number of booster injections, and that the majority of this immunoreactivity was due to cross-reactivity.

Octopamine in the central nervous system of Oligochaeta: an immunocytochemical and biochemical study

OA appears to have a regulatory role in the central nervous system of Oligochaeta, and certain regions of the neuropil of the ganglia were densely innervated by immunoreactive fibers.

Octopamine-like immunoreactivity in the dorsal unpaired median (DUM) neurons innervating the accessory gland of the male cockroach Periplaneta americana

The combination of the cobalt-filling technique with immunohistochemical mapping of cells suggests an octopaminergic innervation of the musculature of the accessory gland by DUM neurons.

Cytoarchitecture of histamine‐, dopamine‐, serotonin‐ and octopamine‐containing neurons in the cricket ventral nerve cord

  • M. Hörner
  • Biology
    Microscopy research and technique
  • 1999
A comparative neuroanatomical description of the cellular localization of the biogenic amines histamine, dopamine, serotonin and octopamine in the ventral nerve cord of an insect, namely the cricket, Gryllus bimaculatus is provided.

Octopamine immunoreactivity in the fruit fly Drosophila melanogaster

Octopamine immunoreactivity in the adult CNS revealed many additional neurons compared to the larval CNS, indicating that at least a subset of adult octopamine neurons may differentiate during metamorphosis.
...

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