A new small myotoxin from the venom of the prairie rattlesnake (Crotalus viridis viridis)

@article{Griffin1990ANS,
  title={A new small myotoxin from the venom of the prairie rattlesnake (Crotalus viridis viridis)},
  author={P. Griffin and S. Aird},
  journal={FEBS Letters},
  year={1990},
  volume={274}
}
Fast atom bombardment (FAB) mass spectrometry was used to identify a new small myotoxin from the venom of the prairie rattlesnake (Crotalus viridis viridis). FAB mass spectrometry and Edman degradation were used to characterize its structure. This toxin is similar to myotoxin I from C. v. concolor. except that it possesses an additional C‐terminal asparaginyl‐alanine. At 45 residues it is the longest known myotoxin a homolog. A myotoxin of 43 residues, identical to myotoxin I from C. v… Expand
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Characterization of the two myotoxin a isomers from the prairie rattlesnake (Crotalus viridis viridis) by capillary zone electrophoresis and fluorescence quenching studies.
  • D. Nedelkov, A. Bieber
  • Chemistry, Medicine
  • Toxicon : official journal of the International Society on Toxinology
  • 1997
TLDR
The two myotoxin a isomers from the venom of the prairie rattlesnake Crotalus viridis viridis have different isoelectric points, as determined by capillary zone electrophoresis, and fluorescence quenching reveals heterogeneity of the tryptophans, possibly due to different environments. Expand
A simple and rapid method for isolating small myotoxins from rattlesnake venoms.
  • Q. Li, T. Colberg, C. Ownby
  • Biology, Medicine
  • Toxicon : official journal of the International Society on Toxinology
  • 1993
TLDR
Many rattlesnake venoms contain small, highly basic toxins which cause contracture and necrosis of skeletal muscle in experimental animals, which are isolated using a Hydrazide Avidchrom Cartridge. Expand
Evidence for isomerization in myotoxin a from the prairie rattlesnake (Crotalus viridis viridis).
TLDR
Mass analysis by matrix-assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry shows that myotoxin a is present in both RP-HPLC peaks, suggesting that the two resolved forms are conformational isomers. Expand
Regional differences in content of small basic peptide toxins in the venoms of Crotalus adamanteus and Crotalus horridus.
TLDR
The combined results of HPLC, immunodiffusion and electrophoresis analyses of venoms from different geographical regions indicate that the SBP toxin content in the venoms of Crotalus adamanteus,Crotalus horridus, CroTalus scutulatus and Crotalu viridis viridis may vary regionally. Expand
Biological activities of a new crotamine-like peptide from Crotalus oreganus helleri on C2C12 and CHO cell lines, and ultrastructural changes on motor endplate and striated muscle.
TLDR
The effects of helleramine provide further insights and evidence of myotoxic activities of crotamine-like peptides and their possible role in crotalid envenomings. Expand
The nucleotide sequence of the translated and untranslated regions of a cDNA for myotoxin a from the venom of prairie rattlesnake (Crotalus viridis viridis).
TLDR
It is likely that myot toxin a is secreted as the cDNA encodes a signal peptide immediately 5' to the myotoxin a peptide code. Expand
Structural and Biological Characterization of Two Crotamine Isoforms IV-2 and IV-3 Isolated from the Crotalus durissus cumanensis Venom
TLDR
These new crotamine isoforms induced potent blockade of neuromuscular transmission in young chicken biventer cervicis preparation and potent myotoxic effect in mice, modulated by the presence of positively charged amino acid residues. Expand
Individual expression patterns of myotoxin isoforms in the venom of the snake Bothrops asper.
  • B. Lomonte, E. Carmona
  • Biology, Medicine
  • Comparative biochemistry and physiology. B, Comparative biochemistry
  • 1992
TLDR
One of the bands, corresponding to the lysine-49 phospholipase myotoxin II, was the only isoform present in all individuals studied, suggesting a possible selective pressure for the conservation of this type of protein in the venom of B. asper. Expand
Structure, Function and Biophysical Aspects of the Myotoxins from Snake Venoms
TLDR
The purpose of this review is to describe the structure and function of these myotoxins and to evaluate features they might share which could shed light on the function and structure of snake venom toxins. Expand
Nucleotide sequence of crotamine isoform precursors from a single South American rattlesnake (Crotalus durissus terrificus).
TLDR
A cDNA phage library was constructed from venom glands of a single adult specimen of crotamine-plus Crotalus durissus terrificus (South American rattlesnake) captured in a known region, and it was indicated that Crotamine transcripts were not expressed in the crotamines-minus specimen. Expand
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