A new skin equivalent: keratinocytes proliferated and differentiated on collagen sponge containing fibroblasts.

@article{Maruguchi1994ANS,
  title={A new skin equivalent: keratinocytes proliferated and differentiated on collagen sponge containing fibroblasts.},
  author={Tomoko Maruguchi and Yukiya Maruguchi and Shigehiko Suzuki and Katsuhiko Matsuda and Ken-ichi Toda and Nobuhiko Isshiki},
  journal={Plastic and reconstructive surgery},
  year={1994},
  volume={93 3},
  pages={537-44; discussion 545-6}
}
Three types of artificial skin containing keratinocytic components were prepared and tested for comparison. Keratinocytes were cultured on the artificial skin dermis (collagen sponge) by the air-liquid interface culture method. In order to create continuous keratinocytic layers on the artificial skin dermis, pores of its uppermost layer were filled beforehand with type I collagen gel, Matrigel, or fibroblasts. A band of keratinocytes consisting of two to six cell layers was formed on collagen… CONTINUE READING

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