A new site-specific integration system for mycobacteria.

  title={A new site-specific integration system for mycobacteria.},
  author={Jeffrey P. Murry and Christopher M. Sassetti and Jonathan Lopes Moreira and James M Lane and Eric J. Rubin},
  volume={85 5-6},
Site-specific integration into the mycobacterial chromosome can produce stable transformants useful for understanding pathogenesis. However, gene expression can be problematic at certain sites of integration. We have used the Streptomyces phiC31 integration system to integrate vector DNA into Mycobacterium smegmatis, M. bovis BCG, and M. tuberculosis through site-specific recombination. A single dominant insertion site was found in M. smegmatis, as previously reported. Three different insertion… CONTINUE READING


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