A new simplified assay for the quantitation of theophylline and its major metabolites in urine by high-performance liquid chromatography.

Abstract

A high-performance liquid chromatographic technique is presented for the simultaneous determination of theophylline, 3-methylxanthine, 1-methyluric acid, 1,3-dimethyluric acid, and caffeine in urine using beta-hydroxyethyl theophylline (BHET) as an internal standard. Following centrifugation of the sample, 2 mL of urine was placed onto a Sep-Pak C18… (More)

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@article{Kester1987ANS, title={A new simplified assay for the quantitation of theophylline and its major metabolites in urine by high-performance liquid chromatography.}, author={Mary Beth Kester and Consuelo L. Saccar and Mario L. Rocci and Herbert C. Mansmann}, journal={Journal of pharmaceutical sciences}, year={1987}, volume={76 3}, pages={238-41} }