A new serial transfer explant cell culture system for human prostatic cancer tissues preventing selection toward diploid cells.

@article{Zwergel1998ANS,
  title={A new serial transfer explant cell culture system for human prostatic cancer tissues preventing selection toward diploid cells.},
  author={T. B. H. Zwergel and Hacer Kakirman and Heike Schorr and Bernd Wullich and Gerhard Unteregger},
  journal={Cancer genetics and cytogenetics},
  year={1998},
  volume={101 1},
  pages={16-23}
}
An improved explant cell culture technique to avoid selection of prostatic adenocarcinoma cells toward diploid cells is described. This method is based on 1) histologically characterized tissue explants, 2) the use of polyethylenteraphthalate (PET) membranes as growth surface, which are part of special inserts in six-well-plates to allow 3) cocultivation with heterologous fibroblasts, and 4) coating of the membranes with elements of the extracellular matrix. The main characteristic of this… CONTINUE READING
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