A new sensitive and specific enzyme-linked immunosorbent assay for IgD.

@article{Mosedale2006ANS,
  title={A new sensitive and specific enzyme-linked immunosorbent assay for IgD.},
  author={David E. Mosedale and Manjinder S. Sandhu and Jian'an Luan and Margaret D Goodall and David John Grainger},
  journal={Journal of immunological methods},
  year={2006},
  volume={313 1-2},
  pages={74-80}
}
We have developed a new highly specific ELISA for IgD, and then used it to measure levels of circulating IgD in the serum of 480 un-selected patients from the East Anglia region of UK. The assay is both extremely sensitive and specific, with a minimum detected IgD concentration of 30 pg/ml and more than 10,000-fold specificity for IgD over all other human immunoglobulins. The assay shows linear dilution characteristics with both purified IgD and human serum, and spiking of purified IgD into… CONTINUE READING