A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition

@article{Guescini2008ANR,
  title={A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition},
  author={M. Guescini and D. Sisti and M. Rocchi and L. Stocchi and V. Stocchi},
  journal={BMC Bioinformatics},
  year={2008},
  volume={9},
  pages={326 - 326}
}
  • M. Guescini, D. Sisti, +2 authors V. Stocchi
  • Published 2008
  • Medicine, Computer Science, Biology
  • BMC Bioinformatics
  • BackgroundReal-time PCR analysis is a sensitive DNA quantification technique that has recently gained considerable attention in biotechnology, microbiology and molecular diagnostics. Although, the cycle-threshold (Ct) method is the present "gold standard", it is far from being a standard assay. Uniform reaction efficiency among samples is the most important assumption of this method. Nevertheless, some authors have reported that it may not be correct and a slight PCR efficiency decrease of… CONTINUE READING
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