A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition

@article{Guescini2008ANR,
  title={A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition},
  author={Michele Guescini and Davide Sisti and Marco B. L. Rocchi and Laura Stocchi and Vilberto Stocchi},
  journal={BMC Bioinformatics},
  year={2008},
  volume={9},
  pages={326 - 326}
}
Real-time PCR analysis is a sensitive DNA quantification technique that has recently gained considerable attention in biotechnology, microbiology and molecular diagnostics. Although, the cycle-threshold (Ct) method is the present "gold standard", it is far from being a standard assay. Uniform reaction efficiency among samples is the most important assumption of this method. Nevertheless, some authors have reported that it may not be correct and a slight PCR efficiency decrease of about 4% could… CONTINUE READING