A new process for obtaining hydroxytyrosol using transformed Escherichia coli whole cells with phenol hydroxylase gene from Geobacillus thermoglucosidasius.

@article{OrenesPiero2013ANP,
  title={A new process for obtaining hydroxytyrosol using transformed Escherichia coli whole cells with phenol hydroxylase gene from Geobacillus thermoglucosidasius.},
  author={Esteban Orenes-Pi{\~n}ero and Francisco Garc{\'i}a-Carmona and Alvaro S{\'a}nchez-Ferrer},
  journal={Food chemistry},
  year={2013},
  volume={139 1-4},
  pages={377-83}
}
Phenol hydroxylase gene cloning from the thermophilic bacteria Geobacillus thermoglucosidasius was used to develop an effective method to convert tyrosol into the high-added-value compound hydroxytyrosol by hydroxylation. Phenol hydroxylase is a two-component enzyme encoded by pheA1 and pheA2 genes and strictly dependent on NADH and FAD. These two genes were subcloned together as a 2 kb fragment into Escherichia coli Rosetta cells, and the transformants were able to grow and effectively… CONTINUE READING
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