A new plasmidic cefotaximase in a clinical isolate of Escherichia coli.


Escherichia coli GRI was isolated from an ear exudate of a newborn. The strain was highly resistant to cefotaxime (MIC 128 mg/l). Resistance to cefotaxime and the majority of beta-lactam antibiotics was readily transferred to an Escherichia coli recipient strain. Both the wild type and the transconjugant strains are different in their resistance phenotype from TEM-3 beta-cefotaximase producers by higher MICs to the majority of beta-lactams and lower MICs to ceftazidime. The isoelectric point of the cefotaximase of E. coli GRI was 8.9 in comparison with 6.3 for TEM-3. Thus, the enzyme produced by E. coli GRI represents a new plasmidic (plasmid pMVP-3) broad spectrum beta-lactamase (CTX-M) which may not be closely related to either the TEM- oder SHV-family of extended broad spectrum beta-lactamases.

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@article{Bauernfeind1990ANP, title={A new plasmidic cefotaximase in a clinical isolate of Escherichia coli.}, author={Adolf Bauernfeind and Helvi Grimm and Samuel A. Schweighart}, journal={Infection}, year={1990}, volume={18 5}, pages={294-8} }