A new phagemid vector for positive selection of recombinants based on a conditionally lethal barnase gene.

@article{Yazynin1999ANP,
  title={A new phagemid vector for positive selection of recombinants based on a conditionally lethal barnase gene.},
  author={S Yazynin and Heike Lange and T Mokros and Sergey M. Deyev and H. Lemke},
  journal={FEBS letters},
  year={1999},
  volume={452 3},
  pages={
          351-4
        }
}
A new phagemid cloning vector for positive selection of recombinants, pBa-7, was constructed which contains an active barnase gene encoding the cytotoxic ribonuclease from Bacillus amyloliquefaciens, under control of the lac promoter. PBa-7 is a derivative of the high-copy number pBluescript II KS+ phagemid in which the modified barnase killer gene has been fused downstream from the lac promoter of the pBluescript II KS+ multiple restriction site. When a lacIq-negative Escherichia coli strain… CONTINUE READING

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