A new pathway in the generation of defective retrovirus DNA.

@article{Olsen1985ANP,
  title={A new pathway in the generation of defective retrovirus DNA.},
  author={John C. Olsen and R. Swanstrom},
  journal={Journal of virology},
  year={1985},
  volume={56 3},
  pages={779-89}
}
We used a retrovirus shuttle vector to make molecular clones of circular viral DNA from infected cells. One-third of the molecules examined had deletions that started within or near the U5 domain of the long terminal repeat (LTR) region and extended a variable distance toward the gag gene. We present evidence that some of these deletions arose by cleavage of a single LTR unit, in contrast to the cleavage of tandem LTR units associated with the integration reaction. These results suggest that in… CONTINUE READING