A new modified DNA enzyme that targets influenza virus A mRNA inhibits viral infection in cultured cells.

@article{Takahashi2004ANM,
  title={A new modified DNA enzyme that targets influenza virus A mRNA inhibits viral infection in cultured cells.},
  author={Hitoshi Takahashi and Hiroyuki Hamazaki and Yuichiro Habu and Mieko Hayashi and Takayuki Abe and Naoko Miyano-Kurosaki and Hiroshi Takaku},
  journal={FEBS letters},
  year={2004},
  volume={560 1-3},
  pages={69-74}
}
DNA enzymes are RNA-cleaving single-stranded DNA molecules. We designed DNA enzymes targeting the PB2 mRNA translation initiation (AUG) region of the influenza A virus (A/PR/8/34). The modified DNA enzymes have one or two N3'-P5' phosphoramidate bonds at both the 3'- and 5'-termini of the oligonucleotides, which significantly enhanced their nuclease resistance. These modified DNA enzymes had the same cleavage activity as the unmodified DNA enzymes, determined by kinetic analyses, and reduced… CONTINUE READING

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