A new micellar aqueous two-phase partitioning system (ATPS) for the separation of proteins.

Abstract

Partitioning of six typical globular proteins with molecular weights ranging from 12.6 to 250 kDa was investigated using an aqueous two-phase system formed by heating a solution containing the individual proteins and n-dodecyldimethylphosphine oxide (APO12) above the cloud point of the nonionic surfactant (approximately 40 degrees C). The partition… (More)

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